|CAI||Codon Adaptation Index|
|HEG||Highly Expressed Genes|
|NGenes||Number of genes|
|RPG||Ribosomal Protein Genes|
|RSCU||Relative Synonymous Codon Usage|
|tRNA||tRNA Gene Copy Number (or tGCN)|
The methods for determining whether a genome is under translational selection and predicting highly expressed genes are described in the following article: Puigbo P, Guzman E, Romeu A and Garcia-Vallve S. OPTIMIZER: A web server for optimizing the codon usage of DNA sequences. Nucleic Acids Research. 2007. Vol. 35, No. suppl_2 W126-W131.
To make the prediction of translational selection, we checked whether the mean position of the ribosomal protein genes along any of the four principal axes (Axis1, Axis2, Axis3 and Axis4) was significantly different (evaluated with a t-test - ns/*:<0.90/0.90 - **:0.95, ***:0.99, ****:0.9995) from the mean position of the other genes of their genome.